Test Directory

Test Code
TESFC
Test Name
Testosterone, Free (Females and Children)
Expected Turnaround Time
1 - 5 days
Clinical Utility
Provides a calculated value for free testosterone concentration using total testosterone measured by mass spectrometry. May be used to evaluate hyperandrogenism in children and cisgender females, monitor testosterone-suppressing hormone therapies (eg, antiandrogens or estrogens), evaluate testosterone status in individuals with protein-binding abnormalities, or evaluate hypogonadism in cisgender males.
Specimen and Container Info

Preferred Specimen Type: Serum

Alternate Specimen Type: Plasma

Preferred Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Green Top Tube (Sodium Heparin); OR Green Top Tube (Lithium Heparin)

Desired Specimen Volume: 1 mL

Collection Instructions

Collect specimen in gold top, serum separator tube; green top (sodium heparin) tube; or green top (lithium heparin) tube via venipuncture. Centrifuge and transfer serum or plasma to plastic aliquot tube.

Processing and Submission Instructions
Collect between 6-10 a.m.
Transport Instructions

Transport to the laboratory refrigerated. 

Stability Requirements

Room Temperature

24 hours

Refrigerated

1 week

Frozen

6 months
Causes for Rejection
EDTA plasma.
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay/Calculation
Reference Intervals

See patient report.

Reference Lab
ARUP Laboratories
Testing Schedule
Routine, daily