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Test Code

TOBR

Test Name

Tobramycin Random

CPT Codes

80200

Expected Turnaround Time

Same day

Clinical Utility

Useful for monitoring adequate drug clearance during therapy, adequacy of blood concentration and assessing toxicity

Specimen and Container Info

Specimen	Container	Volume
Serum	Red Top, Plain	1 mL
Serum	Gold Top, Serum Separator	1 mL
Serum	Microtainer, Red Top	500 µL
Serum	Microtainer, Serum Separator	500 µL
Plasma	Lavendar Top (EDTA)	1 mL
Plasma	Grey Top (Sodium Fluoride, Potassium Oxalate)	1 mL
Plasma	Dark Green Top (Lithium Heparin)	1 mL
Plasma	Microtainer, Lavender Top (EDTA)	500 µL
Plasma	Microtainer, Green Gel	500 µL

Collection Instructions

Random: Collect 6-14 hours after the last dose.

Processing and Submission Instructions

Centrifuge for 5 minutes at 3000 rpm, then transfer to plastic aliquot tube and refrigerate, if specimen will be tested immediately.
If specimen will not tested immediately, the aliquot should be frozen.
Once frozen, transport specimen submerged in dry ice.

Please Note: Concentrations of ß-lactam antibiotic (Penicillns and Cephalosporins) at therapeutic levels may inactivate Tobramycin in vivo and in vitro. Analyze Tobramycin specimens containing ß-lactam antibiotic immediately upon receipt. Freezing the specimen prevents in vitro inactivation and low quantitation.

Transport Instructions

Transport to the laboratory on dry ice.

Methodology

Enzyme Immunoassay (EIA)

Reference Intervals

Age	Reference Interval	Units
All Ages	4.0 - 10.0	µg/mL

Critical Values

Age	Critical Value	Units
All Ages	>=10.1	µg/mL

Performing Location

Roble Road Core Laboratory

Alternate Names

Nebcin

Testing Schedule

Routine daily, STAT testing available