Test Directory

This is a quantitative EIA assay for the human cytokine Transforming Growth Factor beta 1 ( TGF ß-1). TGF β-1 plays a role in tissue regulation and is used as an aid to determine underlying causes of unusual or inflammatory disorders. TGF β-1 is a pleotropic cytokine that possesses both inflammatory and immune-suppressive characteristics. It is a secreted protein that performs many cellular functions, including control of cell growth, proliferation, differentiation and apoptosis.

Specimen Type: Platelet Poor Plasma (EDTA)

Collection Container: Lavender Top (EDTA)

Desired Specimen Volume: 1 mL

1. Collect a lavender top (EDTA) tube via venipuncture.

1. Separate plasma from cells within 30 minutes of collection.
2. Centrifuge at 1,000 X g for 15 min.
3. Transfer top 2/3 of plasma into a clear aliquot tube using a pipette.
4. Centrifuge plasma a second time at 3.000 X g for 10 minutes.
5. Collect the platelet poor plasma above the pellet using a pipette and transfer 1 mL of platelet poor plasma to a LabCorp PP transpak frozen purple tube.

Transport to the laboratory frozen. 

• Grossly lipemic samples

See patient report.

TGF-b1 results are directly affected by sample quality. Compromised samples can produce falsely elevated results. Samples must be collected by venipuncture and processed to be platelet poor. Indicators of poor sample quality or improper processing may include hemolysis.