Test Directory

• ABO (Pre and Post)
• Rh (D) (Pre and Post)
• Direct Antiglobulin Test (Pre and Post)
• Examination of plasma
• Clerical check
• Interpretation

Reflexed when appropriate:

• Antibody Identification
• Phenotype Antigen Test
• Direct Coombs Test
• Eluate
• Antibody Titer
• EGA Treatment
• Thermal Amplitude Test
• Human Erythrocyte Antigen (Genotype)
• IGG IGM Subclass
• Adsorption
• DTT

ABO and Rh (D) type of pre- and post-transfusion specimens is performed. Examination of all clerical work for possible error; examination of pre-reaction and post-reaction serum or plasma for hemolysis and icterus. Direct Antiglobulin Test on pre and post-reaction specimen is included.

Preferred Specimen Type: Whole Blood, Urine

Preferred Collection Container: 1 Pink Top (EDTA), Urinalysis Collection Tube (yellow cap)

Alternate Collection Container: 2 Lavender Top (EDTA)

Desired Specimen Volume: 6 mL Whole Blood and 4 mL urine

Minimum Acceptable Volume: 3 mL Whole Blood and 1 mL urine

Other Instructions: Minimum volume for infants <4 months is 2 full Microtainer, Lavender Top (EDTA) and 1 mL urine

Lehigh Valley Health Network

The specimen tube shall be properly labeled with the following 3 independent identifiers for inpatients and outpatients:

1. Patient's full name
2. Date of birth
3. Medical record number

The labels shall also include the following verifiers:

1. Date and time of collection
2. Initials/tech code of phlebotomist

Exception Criteria:

• Trauma -ED patient: Patients with unknown name and date of birth, medical record number and temporary name are acceptable.
• Good Shepherd Rehab: Typenex band ID is used as an additional patient identifier

Redeemer Health

The specimen shall be identified at the time of collection and labeled at bedside with:

1. Full name of patient exactly as it appears on the patient's identification bracelet
2. Medical record number
3. Phlebotomy code of the person who drew the specimen. If they do not have a code, full name printed legibly.
4. Date and time of collection

Unique patient identifiers (name and medical record number) must not be changed or added once the tube has been labeled at the patient's bedside.

Hospitalized patients: Submit specimens and blood unit with attached IV tubing (remove needles) with a Complete Report of Alleged Transfusion Reaction Form (HNL-51) in EPIC.

Non-hospitalized patients: Submit specimen with a completed Blood Bank Requisition (LAB-04) Form. Transfusion and pregnancy history within the last 3 months should be included.

Minimum Volume for infants <4 months is 1 full lavender top (K2 EDTA) microtainer tubes

Transport immediately

• Mislabeled
• Insufficient volume
• Inappropriate specimen type