Test Directory

• Vedolizumab Quantitation
• Vedolizumab Antibodies

Useful for assessing for primary or secondary loss of response to therapy with vedolizumab and as an aid to achieving desired serum concentrations of vedolizumab.

Specimen Type: Serum

Preferred Collection Container: Red Top, Plain

Alternate Collection Container: Gold Top, Serum Separator

Desired Specimen Volume: 1.5 mL

1. Collect a gold top, serum separator tube via venipuncture.
2. Draw blood immediately before next scheduled dose (trough specimen).

1. Separate serum from cells within 2 hours of collection.
2. Transfer 1.5 mL of serum to a clear aliquot tube (screw cap).

• For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
• Nivolumab (Opdivo) must be discontinued at least 4 weeks prior to testing for vedolizumab quantitation in serum.
• Draw blood immediately before next scheduled dose (trough specimen).

Transport to the laboratory refrigerated. 

See patient report.

Patients actively undergoing therapy with both vedolizumab and nivolumab (rare scenario) should not have their therapeutic vedolizumab concentration assessed using this test. If the patient has taken nivolumab in the past, they should wait for 4 weeks after therapy with nivolumab has ended before being tested for vedolizumab quantitation using this method.

Samples containing more than 100 ng/mL biotin (vitamin B7) may interfere with ATV (in the form of depressed signal) for VEMAB / Vedolizumab Antibodies, Serum.

• Vedolizumab Quantitation with Antibodies, Serum
• VEDOZ
• VEMAB
• Vedolizumab
• VEDO
• Entyvio