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Test Code
VD1
Test Name
Vitamin D, 1,25-Dihydroxy
CPT Codes
82652
Included Tests
  • Vitamin D, 1,25-Dihydroxy
Expected Turnaround Time
3 - 5 days
Clinical Utility

Can be used as a second-order test in the assessment of vitamin D status, especially in patients with renal disease and for the differential diagnosis of hypercalcemia. Can also be used for investigation of some patients with clinical evidence of vitamin D deficiency (e.g. vitamin D dependent rickets due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxy vitamin D).
Specimen and Container Info

Specimen Type: Serum

Preferred Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Red Top, Separator

Minimum Acceptable Volume: 1.0 mL
Processing and Submission Instructions

Separator tubes: Contains clot activator and gel for separating serum from cells, but not anticoagulant. Separate serum from cells as soon as the clot has formed (30 minutes). If specimen is centrifuged before clotting is complete, a fibrin clot will form on top of the cell. This may cause the gel barrier to not be intact and could cause improper separation of serum and cells, possibly affecting test results.
Transport Instructions

Transport to the laboratory refrigerated.
Stability Requirements

Room Temperature

7 days

Refrigerated

14 days
Causes for Rejection
Hemolyzed or lipemic samples are unacceptable for patient testing.
Methodology
Chemiluminescent Immunoassay
Reference Intervals

HNL Lab Medicine Special Chemistry Reference Intervals
Performing Location
Roble Road Core Laboratory
Testing Schedule
Routine, 2 times per week
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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