Test Directory

Test Code
VTD
Test Name
Vitamin D, 25-OH, Total
CPT Codes
82306
Expected Turnaround Time
1 - 3 days
Clinical Utility

Can be used for the diagnosis of vitamin D deficiency, differential diagnosis of Rickets, Osteomalacia, and monitoring Vitamin D replacement therapy.

Specimen and Container Info

Specimen Type: Serum

Preferred Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Red Top, Separator

Minimum Acceptable Volume: 0.8 mL

Processing and Submission Instructions

Separator tubes: Contains clot activator and gel for separating serum from cells, but not anticoagulant. Separate serum from cells as soon as the clot has formed (30 minutes). If specimen is centrifuged before clotting is complete, a fibrin clot will form on top of the cell. This may cause the gel barrier to not be intact and could cause improper separation of serum and cells, possibly affecting test results.

Transport Instructions

Transport to the laboratory refrigerated.

Stability Requirements

Refrigerated

5 days
Causes for Rejection
Grossly hemolyzed or lipemic samples are unacceptable for patient testing.
Methodology
Chemiluminescent Immunoassay
Reference Intervals
Performing Location
Roble Road Core Laboratory
Testing Schedule
Routine, daily