Test Directory

Test Code
WNVSF
Test Name
West Nile Virus RNA, Cerebrospinal Fluid
CPT Codes
87798
Expected Turnaround Time
1 - 5 days
Clinical Utility

Rapid testing for West Nile virus (WNV) RNA (lineage 1 and lineage 2) using cerebrospinal fluid specimens.

An adjunctive test to serology for detection of early WNV infection (ie, first few days after symptom onset).

This assay should not be used for screening asymptomatic individuals and should only be used to test patients with signs and symptoms of WNV disease.

Specimen and Container Info

Specimen Type: Cerebrospinal Fluid

 

Collection Container: Sterile, Non-Additive Container (CSF) 

Desired Specimen Volume: 0.5 mL

Collection Instructions
  1. Collect cerebrospinal fluid (CSF) via lumbar puncture. Collection from vial #2 is preferred.
Processing and Submission Instructions
  1. Transfer 0.5 mL of CSF to a sterile aliquot tube (screw cap).
  2. Do not centrifuge.
Transport Instructions

Transport to the laboratory refrigerated. 

Stability Requirements

Refrigerated

7 days

Frozen

7 days
Causes for Rejection
  • Heat-inactivated specimens
Methodology
Real Time Polymerase Chain Reaction
Reference Intervals

See patient report.

Limitations

A negative test does not exclude infection with West Nile virus. Therefore, the results obtained should be used in conjunction with clinical findings and serologic test results to make an accurate diagnosis.

This assay detects both viable and nonviable virus. Test performance depends on viral load in the specimen and may not correlate with cell culture performed on the same specimen.

Alternate Names
  • Arbovirus
  • Flavivirus
  • Mosquito Borne Encephalitis
  • Viral encephalitis
  • West Nile PCR
  • West Nile Virus (WNV)
  • WNV PCR
Reference Lab
Mayo Clinic Laboratories
Testing Schedule
Monday-Friday